New England Biolabs, S1402S, Ribonucleoside Vanadyl Complex

Related Categories RNase Inhibitors Specification Shipping Notes Ships on dry ice FAQ Q: For how long should I heat at 65°C? A: The sample should be heated until it is fully thawed and homogenous. To reduce oxygen exposure, keep the vial sealed until completely thawed and ready for aliquoting. Q: There is some solid in my Ribonucleoside Vanadyl Complex, is that okay? A: Yes. Sodium sulfate is generated as a byproduct of the complexation reaction and may be present as a minor insolubility depending on solution temperature. Q: Can I use Ribonucleoside Vanadyl Complex for in vitro transcription (IVT) reactions, or in other enzymatic workflows? A: RVC is known to have an inhibitory effect on many enzymatic reactions, so we generally discourage its use in this context, recommending other ribonuclease inhibitors that NEB offers instead – Rnase Inhibitor, Human Placenta (NEB #M0307), and RNase Inhibitor, Murine (NEB #M0314). In some cases, however, the inhibitory effects of RVC have been managed through a reduction of RVC concentration relative to other reaction components (5, 6).