New England Biolabs, S1515S, NEBNext® Magnetic Separation Rack

Related Categories FFPE DNA,, Microbiome DNA Enrichment,, DNA Library Prep for Illumina, FAQ Q: What are the dimensions and weight of the NEBNext® Magnetic Separation Rack? A: Length: 109 mm Height: 21.49 mm Width: 38.1 mm Approximate weight: 0.42 lbs Q: What types of tubes, and how many tubes, does the NEBNext Magnetic Separation Rack hold? A: The separation rack accepts 8- and 12-strip 0.2 ml PCR tubes or individual 0.2 ml PCR tubes, with a total capacity of up to 24 tubes. Q: What is the minimum volume for separation? A: The minimum volume for separation is 5 μl. Note that you may need to push the tube against the magnet to achieve optimal separation at this low volume. Q: What is the optimal temperature for use? A: Room temperature is optimal. Do not expose the magnetic separation rack to extremely high temperatures, above 80°C (175°F), as the magnets will lose their magnetic properties. Q: Can the rack accommodate tubes with caps? A: Yes, the NEBNext Magnetic Separation Rack can accommodate standard 0.2 ml PCR strip tubes and individual 0.2 ml PCR tubes with caps. Q: Are there any specific recommendations when using the NEBNext Magnetic Separation Rack with smaller volumes? A: When working with smaller volumes, more efficient separation occurs if you gently push the tube against the magnet (note that this adjustment will require a rise in tube position). Q: Is the NEBNext Magnetic Separation Rack compatible with individual 0.2 µl PCR tubes? A: Yes, the NEBNext Magnetic Separation Rack is compatible with individual 0.2 µl PCR tubes. Q: What is a recommended procedure if unwanted beads are retained in the pipet tips during an elution step? A: Gently expel the entire contents of the pipet tip into the original tube(s) and allow an additional incubation on the magnet for 1 minute, or longer if necessary. Attempt elution again, carefully positioning the pipet tips on the wall of the tube(s) facing away from the magnet center and bound beads. Note: A slower pipet aspiration, or multi aspiration with smaller volumes, may be useful in this situation. Q: What is the optimal volume for separation? A: The optimal volume for separation is 5 μl to 100 μl of beads, with a maximum total volume in the tube of 200 μl.