New England Biolabs, S9102S, SNAP-Cell® 647-SiR

SNAP-Cell Related Categories SNAP-tag Substrates ,, Protein Labeling Applications SNAP Cell,, In vivo, Imaging,, Pulse Chase, Specification Materials Required but not Supplied Cells expressing SNAP-tag fusion proteins Tissue culture materials and media Transfection reagents Fluorescence microscope with suitable filter set DMSO Anti-SNAP-tag® Antibody (Polyclonal) (NEB #P9310) (optional) SNAP-tag® Purified Protein (NEB #P9312) (optional) SNAP-Cell® Block (NEB #S9106) (optional) Excitation 645nm Emission 661nm FAQ Q: Cellular Imaging and Analysis FAQs A: View all Cellular Imaging and Analysis FAQs. Q: We have recently ordered the SNAP-Cell 647-SiR and when it was resuspended in DMSO the solution didn’t change to a blue color like SNAP-Surface 649 and SNAP-Surface Alexa Fluor 647. Is there something wrong with the substrate? A: When SNAP-Cell 647-SiR is dissolved in DMSO, it remains colorless, unlike SNAP-Surface 649 or SNAP-Surface Alexa Fluor 647 which turn a blue color upon reconstitution in DMSO. The color of the fluorescent substrate upon dissolution in DMSO is not an indicator of its performance.