New England Biolabs, T1121L, Monarch® Buffer BY

Related Categories DNA Gel Extraction,, Nucleic Acid Purification Applications Nucleic Acid Purification FAQ Q: What is the composition of Monarch Buffer BY (NEB #T1121)? A: The composition of the buffers is proprietary. We can, however, share that the Monarch Buffer BY is a guanidine thiocyanate-based buffer. Q: Which kit includes the Monarch Buffer BY (NEB #T1121)? A: Buffer BY is included in the Monarch Spin DNA Gel Extraction Kit (NEB #T1120). Q: Can I use Monarch Buffer BY (NEB #T1121) with the Monarch DNA Gel Extraction Kit (NEB #T1020)? A: Yes, the Monarch Buffer BY is compatible with the Monarch DNA Gel Extraction Kit (NEB #T1020), which has been discontinued. It serves as a gel-dissolving buffer. Q: I see precipitation in Buffer BY (NEB #T1121); can I still use the buffer? A: Yes, if a precipitate has formed in Monarch Buffer BY, incubate the buffer at 30-37ºC, inverting periodically to dissolve. This does not impact the buffer’s functionality or DNA yield.