New England Biolabs, T1157L, Monarch® Spin Columns S3A and Tubes

Related Categories PCR & DNA Cleanup,, Nucleic Acid Purification FAQ Q: Are Monarch® Spin Columns S3A (NEB #T1157) compatible with vacuum manifolds? A: Yes, Monarch Spin Columns S3A are compatible with vacuum manifolds. Please refer to protocols using vacuum manifolds provided in the manual. Vacuum manifolds can be utilized with some other Monarch columns but may require optimization and/or additional time for the liquid to completely flow through. Q: Are the Monarch® Spin Columns S3A (NEB #T1157) compatible with the QIAcube® system? A: The new Monarch spin columns and collection tubes are engineered to ensure high-quality nucleic acid purification. They feature a low elution volume, no buffer retention, and reduced plastic usage. However, these new columns are not compatible with the QIAcube system. Q: Can I use water to elute the DNA when using Monarch® Kits? A: Yes, water can be used to elute DNA from Monarch columns. For maximum elution efficiency, ensure the water is nuclease-free. If you are storing the DNA long-term, we recommend using the supplied Monarch Buffer EY (DNA elution buffer), containing 0.1 mM EDTA. Q: What is the smallest volume of elution buffer that can be used with the Monarch® Spin Columns S3A (NEB #T1157)? A: The range for elution volume using Monarch Spin Columns S3A with Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) is 50 - 200 μl, with 100 μl as a recommended elution volume. When using minimum elution volume, it is recommended to incubate at room temperature for a few more minutes than recommended to maximize yield.