New England Biolabs, T2042L, Monarch® Buffer WX

A buffer designed for wash steps in nucleic acid purification. Included in Monarch Nucleic Acid Purification kits. Related Categories RNA Cleanup,, RNA Extraction and Purification,, Nucleic Acid Purification FAQ Q: What is the composition of each buffer provided with the Monarch Spin RNA Cleanup Kits? A: The compositions of the buffers are proprietary. We can, however, share the following: Monarch Buffer BX....................Guanidine-based binding buffer Monarch Buffer WX.......................Ethanol-based wash buffer Q: What factors affect my (A260/A230) when using the Monarch Spin RNA Cleanup Kits? A: Guanidine and ethanol, both introduced during the prep, can reduce the A260/A230 ratio. Following the protocol, especially the wash steps, will ensure these components are removed. Q: Can I purchase Monarch® buffers and columns separately? A: Monarch spin columns and several Monarch buffers and reagents are also offered separately. Q: Can extraction of RNA with the Monarch kits be automated? A: Yes. NEB has successfully automated the extraction of total RNA (including viral RNA) from saliva samples on the KingFisher™ Flex automation platforms using the workflows for both the Monarch Total RNA Miniprep Kit (NEB #T2010) and the Monarch RNA Cleanup Kit (NEB #T2040), with some protocol modifications. When automated on the KingFisher Flex platform, spin columns are not utilized; instead, magnetic beads (e.g. Dynabeads® MyOne™ Silane magnetic beads) are required. For the purpose of viral RNA extraction, when using the Monarch RNA Cleanup Kit workflow, an added step using Monarch DNA/RNA Protection Reagent (NEB #T2011), sold separately, is required. Our detailed protocols contain more information: Automation of RNA Extraction from Saliva Using the Monarch Total RNA Miniprep Kit Automation of RNA Extraction from Saliva Using the Monarch RNA Cleanup Kit Q: Can the Monarch Spin RNA Cleanup Kits be used for RNA extraction? A: Yes. Although originally developed for RNA cleanup applications, these kits can also be used for RNA extraction from cells, saliva, and swab samples. When used for viral extraction, the Monarch DNA/RNA Protection Reagent (sold separately, NEB #T2011) must be used for viral inactivation. Visit our online protocols for more information: RNA Extraction from Cells Using the Monarch Spin RNA Cleanup Kits RNA Extraction from Buccal/Nasopharyngeal Swabs Using the Monarch Spin RNA Cleanup Kits RNA Extraction from Saliva Using the Monarch Spin RNA Cleanup Kits Automation of Viral RNA Extraction from Saliva Using the Monarch Spin RNA Cleanup Kit Q: I noticed the buffer names have changed in the RNA Cleanup Kits. Are these buffers the same formulation as before? A: Yes NEB is standardizing Monarch buffer naming nomenclature. The buffer names are changing, but the buffer formulations are the same. The Monarch® RNA Cleanup Binding Buffer is now named Monarch® Buffer BX. The Monarch® RNA Cleanup Wash Buffer is now named Monarch® Buffer WX.