New England Biolabs, T2104S, Monarch® DNase I, Lyophilized

Related Categories Total RNA Extraction & Purification,, DNA Modifying Enzymes & Cloning Technologies,, Nucleic Acid Purification Applications Nucleic Acid Purification,, Cloning & Synthetic Biology Specification Materials Required but not Supplied Nuclease-free Water (NEB #B1500) FAQ Q: What is the difference between Monarch DNase I, Lyophilized (NEB #T2104) and DNase I, (RNase-free) (NEB #M0303)? A: Monarch DNase I, Lyophilized (NEB #T2104) is a lyophilized form of DNase I (RNase-free) (NEB #M0303). It is stable at room temperature prior to rehydration. Both are RNase-free formulations and follow the same measurement for unit activity . DNase I (RNase-free) (NEB #M0303) is supplied as a solution of 2,000 U/ml, and Monarch DNase I, Lyophilized (NEB #T2104) is supplied as a dry cake containing 2,200 U of DNase. Q: How do I store the Monarch DNase I, Lyophilized (NEB #T2104) upon receiving it? A: Monarch DNase I, Lyophilized (NEB #T2104) is stable at room temperature for up to 3 years prior to rehydration. Q: How do I store Monarch DNase I, Lyophilized (NEB #T2104) after rehydration? A: After rehydration with nuclease-free water, store at –20°C in appropriately sized aliquots to limit freeze-thaw cycling. Q: How many freeze-thaws can the rehydrated Monarch DNase I, Lyophilized (NEB #T2104) tolerate? A: We recommend no more than 3 freeze-thaws for the rehydrated enzyme. Q: What volume should I rehydrate in to use with Monarch Spin RNA Isolation Kit (Mini) (NEB #T2110)? A: For use with the Monarch Spin RNA Isolation kit, add 550 µl nuclease-free water to the lyophilized cake (NEB #T2104-1). This results in a concentration of 4 U/µl enzyme. Q: What volume should I rehydrate in to make it equivalent to NEB’s liquid DNase product NEB #M0303? A: Add 1,100 µl nuclease-free water to the lyophilized cake (NEB #T2104-1). This will result in a concentration of 2 U/µl, equal to NEB #M0303. Q: I want a concentrated form of DNase that is higher than the typical range. What is the minimum volume for rehydration of Monarch DNase I, Lyophilized (NEB #T2104)? A: We recommend rehydrating in at least 100 µl nuclease-free water.