New England Biolabs, T2110S, Monarch® Spin RNA Isolation Kit (Mini)

Related Categories Total RNA Extraction & Purification,, RNA Extraction and Purification,, Nucleic Acid Purification FAQ Q: How can I assess RNA integrity and purity? A: The purity of eluted RNA samples can be quickly assessed by reviewing OD ratios collected during routine spectrophotometry. Pure RNA typically has an A260/280 of 1.9–2.1, and an A260/230 of 2.0–2.2. Many factors can influence these values such as the use of a proper reference blank solution, the buffer pH, and contaminants such as protein, buffer salts, ethanol, etc. For further information on this topic, please see Assessing RNA. Q: Can components from the older Monarch® Total RNA Miniprep Kit (NEB #T2010) be used interchangeably with those from the new version of the kit (Monarch Spin RNA Isolation Kit (Mini), NEB #T2110)? A: Yes, while the new version of the Monarch Spin RNA Isolation Kit (Mini) is developed with improvements to both the physical column and buffer chemistry, the previous version's components are compatible with the updated kit. Please reach out to NEB Tech Support if component-specific guidance is needed. Q: Are samples stored in DNA/RNA Protection Reagent compatible with the new version of the kit (Monarch Spin RNA Isolation Kit (Mini), NEB #T2110)? A: Yes, samples preserved DNA/RNA Protection Reagent can be processed with the Monarch Spin RNA Isolation Kit (Mini). We recommend proceeding with mechanical homogenization and/or Proteinase K in the DNA/RNA Protection Reagent as recommended in Monarch® Total RNA Miniprep Kit (NEB #T2010) protocol, and replacing RNA Lysis Buffer addition with Monarch StabiLyse DNA/RNA Buffer to continue with the new kit's protocol. Q: I see some silica residue in my Monarch column. Is this a problem? A: No, this is not an issue. The presence of some silica residue or particles in the nucleic acid purification column does not impact nucleic acid binding or purity. Our columns are designed to retain silica fibers, helping ensure they do not end up in the final eluate. Q: I need more Proteinase K and DNase enzymes to use with the kit. Can I purchase the enzymes separately? A: NEB offers standalone products for both Monarch® DNase I, Lyophilized (NEB #T2104) and Proteinase K, Molecular Biology Grade (NEB #P8107). Q: I have used all the Proteinase K (NEB #P8200) included in the Monarch kit. Can I purchase more? A: Yes, Proteinase K, Molecular Biology Grade (NEB #P8107), is available for standalone purchase and can be used as a direct substitute for (NEB #P8200) in all Monarch protocols without any modifications.