New England Biolabs, T4010X, Monarch® Mag Viral DNA/RNA Extraction Kit

The Monarch Mag Viral DNA/RNA Extraction Kit enables reliable, high-throughput purification of viral nucleic acid using a magnetic bead-based protocol. Related Categories Magnetic Bead-Based Nucleic Acid Purification,, Nucleic Acid Purification Applications Nucleic Acid Purification FAQ Q: The enzymes in the kit were not stored at -20°C right away. Will they still work? A: Yes, unopened Monarch Proteinase K and RNAse A enzymes are stable at ambient temperatures short-term. After opening, Proteinase K and Monarch RNAse A should be stored at -20°C. Q: How do I prepare Viral DNA/RNA Wash Buffer? A: Prepare the Viral DNA/RNA Wash Buffer in a user-supplied tube by combining Monarch Buffer BX (supplied), Nuclease-Free Water (supplied) and Isopropanol (user-supplied) as described in the protocol. We recommend that the Viral DNA/RNA Wash Buffer be prepared fresh before the extraction. Q: Does the kit include Nuclease-Free Water to prepare all wash buffers? A: The supplied Nuclease-Free Water is enough for preparing the Viral DNA/RNA Wash Buffer. Users provide their own nuclease-free water for preparing 80% ethanol. To support our sustainability efforts, we have not included additional bottles and nuclease-free water for the 80% ethanol wash steps to avoid shipping excessive materials that may not apply to all users. Q: Where can I find automation scripts? A: For KingFisher platforms, please load the MagMAX script for higher volume and modify the volumes on BindIt interface to match our workflow. For other automation platforms, please contact our technical support for the latest information. Q: What is the sample input volume range? A: The standard workflow is recommended using 200 µL input sample volume. Lower and higher volumes can be used by proportionally changing the Lysis Buffer Bead Mix. Please contact our technical support for further assistance. Q: What sample types are compatible with T4010? A: The kit has been tested for swab and saliva samples. Other samples, such as wastewater, and milk have been evaluated with protocol modifications. Q: What downstream applications are compatible with T4010? A: RT-qPCR, ddPCR, and sequencing. Q: What is the purpose of Carrier RNA, and is it necessary to include in the extraction? A: Adding carrier RNA (poly(A)) to samples during processing can enhance the recovery of low amounts of viral RNA and DNA by acting as a co-precipitant. It also can reduce the likelihood of viral RNA degradation due to RNases by acting as a decoy. Users may omit the use of carrier RNA; however, viral DNA and RNA recovery may be reduced. Carrier RNA should be omitted if the downstream application utilizes a poly(A) RNA enrichment step. Q: I have used all the Proteinase K (NEB #P8200) included in the Monarch kit. Can I purchase more? A: Yes, Proteinase K, Molecular Biology Grade (NEB #P8107), is available for standalone purchase and can be used as a direct substitute for (NEB #P8200) in all Monarch protocols without any modifications.