New England Biolabs, T4130V, Monarch® Mag PCR DNA Cleanup Kit (5 μg)

Related Categories Magnetic Bead-Based Nucleic Acid Purification,, PCR & DNA Cleanup,, Nucleic Acid Purification Applications Nucleic Acid Purification FAQ Q: Can I purchase additional Monarch® Mag Beads M2 for Monarch Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130) separately? A: Yes, magnetic beads (NEB #T4105) for Monarch Mag PCR & DNA Cleanup Kit (5 μg) are also available separately. Q: Can I use water to elute the DNA when using Monarch® Mag PCR & DNA Cleanup Kits (5 μg) (NEB #T4130)? A: Yes, water can be used to elute the DNA. For maximum elution efficiency, ensure the water is nuclease-free and the pH is between 7 and 8.5. Milli-Q™ water is often slightly acidic, requiring pH adjustment. If you are storing the DNA long-term, we recommend using the supplied Monarch Buffer EY (DNA elution buffer), containing 0.1 mM EDTA. Q: Can I use Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130) for size selection? A: The Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130) is designed for purification and cleanup and efficiently removes primers, nucleotides, and other reaction components. However, this kit does not support size-selection applications. Q: Do the magnetic beads in the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130) need to be completely dry before eluting DNA? A: Proper drying of magnetic beads is a critical step to ensure optimal DNA recovery and purity. Beads should be dried just enough to eliminate any visible free-flowing liquid, while still retaining a shiny, dark brown appearance. This indicates they are ready for elution. Avoid over-drying, as it can negatively impact DNA recovery. Over-dried beads typically appear matte and light brown and may be more difficult to resuspend. Q: Is the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130) compatible with an automated workflow? A: Yes, the kit is compatible with automated workflows and instruments. It can be integrated into various liquid handling platforms for high-throughput DNA cleanup. The magnetic bead format allows for easy automation of binding, washing and elution steps. Q: Is the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130) compatible with RNA samples? A: While RNA may be recovered using the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130), the buffers and protocols are not designed for RNase-free conditions or reliable RNA purification. Therefore, we do not recommend using this kit for RNA cleanup. Q: What is the composition of each buffer provided with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)? A: The composition of the buffers is proprietary. We can, however, share the following: Monarch Buffer BZ: Guanidine and isopropanol-based binding buffer Monarch Buffer WZ: Ethanol-based wash buffer Monarch Buffer EY: 10 mM Tris, 0.1 mM EDTA, pH 8.5 elution buffer Q: What is the maximum binding capacity of the Monarch® Mag Beads M2 provided with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)? A: The Monarch® Mag Beads M2 have a binding capacity of up to 5 µg of DNA per prep when using the standard workflow (20 µl of beads) in the DNA cleanup & purification application. This capacity is flexible and can be adjusted by increasing or decreasing the volume of beads used. Protocols are available to support scaling the reaction size up or down, allowing for DNA input either below or above 5 µg, depending on experimental needs. Q: What is the smallest volume of elution buffer that can be used with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)? A: For standard workflows, we recommend an elution volume of 25–50 μl when using this kit. For automated workflows, the same elution range (25–50 μl) can generally be used; however, compatibility depends on the specific automation platform. We recommend checking the instrument’s specifications to ensure the selected volume meets the platform’s minimum tolerable volume requirements. Q: What size DNA can be purified with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)? A: Recovery of DNA from as small as 50 bp to as large as 25 kb can be achieved with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130). Efficiency decreases with longer DNA due to tighter binding to the matrix. The recovery of DNA ≥ 15 kb can be improved by modifying the elution method, using heated elution buffer (50°C) and extending incubation times. For optimal recovery of large DNA fragments, please follow the specific protocol available. Q: What types of DNA samples are compatible with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)? Are there specific protocols available? A: Yes, the kit is compatible with a wide range of DNA types other than linear DNA, including: Oligonucleotides and short DNA fragments: Both single-stranded and double-stranded oligonucleotides can be recovered using this kit. Please refer to the Protocol for Oligonucleotide Cleanup Using the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130). Circular DNA: Both single-stranded and double-stranded circular DNA can be purified using the Protocol for Standard PCR & DNA Cleanup in a Manual Workflow Using the Monarch Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130). Large DNA fragments (>25 kb), including genomic DNA: These can be effectively purified using a Protocol for Genomic DNA (gDNA) Cleanup Using the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130). RCA products: Rolling Circle Amplification (RCA) reactions typically yield a high concentration of DNA and can be purified using the Protocol for RCA Reaction Cleanup Using the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130). For best results, please use the appropriate protocol on our website tailored to your specific sample type. Q: When using the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130), I have residual beads in my eluted DNA. How do I avoid bead carryover? A: Use a strong magnet to ensure efficient separation of magnetic beads. When removing the supernatant, take care not to disturb the bead pellet. If the pellet is accidentally disrupted, repeat the magnetic separation step to allow the beads to fully re-aggregate before proceeding with supernatant removal.