Promega, AX5810, PowerSeq® CRM Nested System, Custom

Produce MPS Libraries Ready for Mitochondrial Control Region Analysis
Massively parallel sequencing of mitochondrial HVI, HVII and HVIII control regions in a single reaction
Nested amplification protocol greatly reduces number of steps and time required for library preparation
Small amplicons ranging from 144–237bp for better results with compromised samples
Specifications：
PowerSeq® CRM Nested 10X Primer Pair Mix AX579A 1 × 250μl
PowerSeq® Nested System Index Primer 1 D701 AX580A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D702 AX582A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D703 AX583A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D704 AX584A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D705 AX585A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D706 AX586A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D707 AX587A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D708 AX588A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D709 AX589A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D710 AX590A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D711 AX591A 1 × 75μl
PowerSeq® Nested System Index Primer 1 D712 AX592A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D501 AX593A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D502 AX594A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D503 AX595A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D504 AX596A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D505 AX597A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D506 AX598A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D507 AX599A 1 × 75μl
PowerSeq® Nested System Index Primer 2 D508 AX600A 1 × 75μl
PowerSeq® 5X Master Mix DP116A 1 × 500μl
Massively parallel sequencing (MPS) allows laboratories access to mitochondrial DNA (mtDNA) analysis using a more sensitive and high-throughput workflow compared to traditional sequencing methods. Increased mixture deconvolution and heteroplasmy resolution are achieved by deep sequencing coverage and digital read counts. Additionally, the use of small amplicons to sequence the mitochondrial control region improves sequencing results from degraded samples.
The PowerSeq® CRM Nested System, Custom, generates 10 small amplicons covering the control region of the mitochondrial genome in one multiplex. The targeted regions for amplification are designed to be in a range of 144–237bp to ensure optimal results from degraded samples. The system uses a nested amplification protocol that greatly reduces the number of steps and time required to produce libraries ready for sequencing. The protocol consists of a single PCR step to both amplify the target amplicons and incorporate indexed sequencing adapters.
Sequencing data can be analyzed using
GeneMarker HTS Software
(SoftGenetics, LLC).