Promega, E1841, pCI-neo Mammalian Expression Vector

Constitutively Express Cloned Insert for Transient or Stable Transfection
Select for stably transfected cells with neomycin phosphotransferase gene
Increase the steady-state level of RNA with the late SV40 polyadenylation signal approximately fivefold more than the early SV40 polyadenylation signal
Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate ssDNA in
E. coli
using the f1 origin of replication