Promega, G9082, NAD/NADH-Glo™ and NADP/NADPH-Glo™ Assays

Bioluminescent Assays to Detect NAD(P)+ and NAD(P)H Levels in Cells
Homogeneous, single-reagent-addition assays
Easily adapted for high-throughput screening formats
Specifications：
Reductase G884A 1 × 55μl
Reductase Substrate G885A 1 × 55μl
NAD Cycling Enzyme (lyophilized) G887A 1 × 1 vial
NAD Cycling Substrate G888A 1 × 1.25ml
Reductase G884B 1 × 275μl
Reductase Substrate G885B 1 × 275μl
NAD Cycling Enzyme (lyophilized) G887A 1 × 1 vial
NAD Cycling Substrate G888A 1 × 1.25ml
Reductase G884A 1 × 55μl
Reductase Substrate G885A 1 × 55μl
NADP Cycling Enzyme (lyophilized) G889A 1 × 1 vial
NADP Cycling Substrate G890A 1 × 275μl
Reductase G884B 1 × 275μl
Reductase Substrate G885B 1 × 275μl
NADP Cycling Enzyme (lyophilized) G889A 1 × 1 vial
NADP Cycling Substrate G890A 1 × 275μl
The NAD/NADH-Glo and NADP/NADPH-Glo Assays are bioluminescent, homogeneous, single-reagent-addition methods to rapidly detect NAD+ and NADH and NADP+ and NADPH levels in cells and enzymatic reactions. Current methods to detect nicotinamide adenine dinucleotides lack sensitivity and reproducibility and require sample manipulation and large numbers of cells for cell-based applications. The assay is easily adapted for inhibitor screening in high-throughput formats. The sensitivity and robustness of the assay chemistry allow fewer cells to be used to detect individual nucleotides directly in multiwell plates or analysis of enzymes with low activity or with low Km values.