Promega, G9953, Caspase-Glo® 1 Inflammasome Assay

A Bioluminescent Method to Directly Measure Inflammasome Activity
Fast, simple, homogeneous caspase-1 assay kit
Selective and quantitative
Multiplex and high-throughput screening (HTS) compatible
Specifications：
Z-WEHD Substrate G991A 1 × 1 bottle
Ac-YVAD-CHO G992A 1 × 10μl
Caspase-Glo® 1 Buffer G994A 1 × 10ml
Z-WEHD Substrate G991A 5 × 1 bottle
Ac-YVAD-CHO G992A 5 × 10μl
Caspase-Glo® 1 Buffer G994A 5 × 10ml
Caspase-Glo® 1 Buffer G994B 1 × 100ml
Z-WEHD Substrate G991B 1 × 1 bottle
Ac-YVAD-CHO G992B 1 × 100μl
The Caspase-Glo® 1 Inflammasome Assay is a simple, homogeneous, bioluminescent method to selectively measure the activity of caspase-1, an essential component of the inflammasome.
Inflammasomes are protein complexes induced by diverse inflammatory stimuli. Innate immune cells respond to pathogens and other danger signals with inflammasome formation and conversion of procaspase-1 zymogen into catalytically active caspase-1. Caspase-1 activation results in the processing and release of cytokines IL-1β and IL-18, as well as pyroptosis, an immunogenic form of cell death. The Caspase-Glo® 1 Assay is sensitive enough to measure caspase-1 activity directly in cells or in medium in multiwell plates.
This peer-reviewed paper "A bioluminescent caspase-1 activity assay rapidly monitors inflammasome activation in cells" shows how the Caspase-Glo® 1 Assay can specifically detect caspase-1 activity in cells treated with inflammasome inducers, confirm one-signal and two-signal inflammasome activation mechanisms, and monitor caspase-1 activity, cell death, and IL-1β release from the same microplate well.
This peer-reviewed study "Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays" examines the direct engagement and functionality of NLRP3 pathway activity in cells using several mechanistic assays, including the
Lumit® IL-1β Immunoassay
and Caspase-Glo® 1 Inflammasome Assay.