Promega, GA1322, TLR Reporter Bioassay

Measure Potency and Stability of Molecules that Modulate TLR Signaling
96- and 384-well plate formats
No primary cell culture required
Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
Specifications：
TLR Bioassay Cells GA132A 1 × 0.5ml
Fetal Bovine Serum J121A 1 × 4ml
RPMI 1640 Medium G708A 1 × 36ml
Bio-Glo-NL™ Luciferase Assay Buffer J308A 1 × 10ml
Bio-Glo-NL™ Luciferase Assay Substrate J309A 1 × 200μl
TLR Bioassay Cells GA132A 5 × 0.5ml
Fetal Bovine Serum J121A 5 × 4ml
RPMI 1640 Medium G708A 5 × 36ml
Bio-Glo-NL™ Luciferase Assay Buffer J308A 5 × 10ml
Bio-Glo-NL™ Luciferase Assay Substrate J309A 5 × 200μl
TLR Bioassay Cells (CPM) GA130A 2 × 1ml
TLR Bioassay Cells (CPM) GA130A 50 × 1ml
The human immune system responds to conserved damage- and pathogen-associated molecular patterns (DAMPs and PAMPs, respectively) via pattern-recognition receptors (PRRs). One such family of PRRs is the Toll-like receptor (TLR) family. In humans, there are 10 known TLRs, which enable the immune system to sense and respond to damage and danger signals. Modulation of the TLR system, either by agonism or antagonism, has the potential to alter immune and inflammatory responses. Blockade of TLRs may inhibit inflammatory responses while TLR agonists may promote immune responses against pathogens or cancer.
Current methods for assessing the activity of TLR modulators typically rely on primary monocyte-derived macrophages and measurement of functional endpoints, such as cytokine production. These assays are laborious and highly variable due to their reliance on donor cells, complex assay protocols and unqualified assay reagents. As a result, these assays are difficult to establish in a quality-controlled setting.
The TLR Bioassay is a bioluminescent reporter cell-based assay used to measure the potency and stability of TLR agonists.