Promega, GA6020, ADCP Reporter Bioassay (THP-1)

Measure Potency and Stability of Biologics that Bind and Activate ADCP through FcγRs
Prequalified according to ICH guidelines
96- and 384-well plate formats
No primary cell culture required
Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
Specifications：
ADCP Bioassay Effector Cells (THP-1) GA128A 1 × 0.5ml
RPMI 1640 Medium G708A 1 × 36ml
Low IgG Serum G711A 1 × 4ml
Bio-Glo-NL™ Luciferase Assay Buffer J308A 1 × 10ml
Bio-Glo-NL™ Luciferase Assay Substrate J309A 1 × 200μl
ADCP Bioassay Effector Cells (THP-1) GA128A 5 × 0.5ml
RPMI 1640 Medium G708A 5 × 36ml
Low IgG Serum G711A 5 × 4ml
Bio-Glo-NL™ Luciferase Assay Buffer J308A 5 × 10ml
Bio-Glo-NL™ Luciferase Assay Substrate J309A 5 × 200μl
ADCP Bioassay Effector Cells (THP-1) (CPM) GA127A 2 × 1ml
ADCP Bioassay Effector Cells (THP-1) (CPM) GA127A 50 × 1ml
Antibody-dependent cellular phagocytosis (ADCP) is an important mechanism-of-action (MOA) for antibody-based immunotherapies. ADCP can be mediated by monocytes, macrophages, neutrophils and dendritic cells via FcγRIIa, FcγRI and FcγRIIIa. In myeloid cells, the expression level of the various receptors is highly dynamic and influenced by cell lineage, tissue microenvironment and local inflammatory state. These receptors can participate in antibody recognition, receptor clustering and signaling events that result in ADCP.
Current methods for measuring ADCP rely on the isolation of primary human monocytes, ex vivo differentiation into macrophages and direct measurement of phagocytosis. These assays are laborious and highly variable due to their reliance on donor cells, complex assay protocols and unqualified assay reagents. As a result, these assays are difficult to establish in quality-controlled, drug development settings.
The ADCP Reporter Bioassay (THP-1) is a bioluminescent reporter cell-based assay used to measure the potency and stability of antibodies and other biologics with Fc domains that bind and activate FcγRs.