Promega, J2272, GITR Bioassay

Measure Potency and Stability of Biologics Designed to Activate GITR
Prequalified according to ICH guidelines
96- and 384-well plate formats
Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
Specifications：
GITR Effector Cells J229A 1 × 0.5ml
Fetal Bovine Serum J121A 1 × 4ml
RPMI 1640 Medium G708A 1 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 1 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 1 × 1 vial
GITR Effector Cells J229A 5 × 0.5ml
Fetal Bovine Serum J121A 5 × 4ml
RPMI 1640 Medium G708A 5 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 5 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 5 × 1 vial
GITR Effector Cells (CPM) J227A 2 × 1ml
Control Ab, Anti-GITR K117A 1 × 50μg
The GITR Bioassays are bioluminescent cell-based assays used to measure the potency and stability of ligands or agonist antibodies that bind and activate GITR. GITR (CD357/TNFRSF18), a member of the tumor necrosis factor (TNF) receptor superfamily, is a costimulatory receptor widely expressed on most immune cells and further upregulated on activated T cells. When engaged with GITR ligand (GITRL) on the cell surface, GITR enhances subsequent T cell expansion and cytokine production including interleukin-2 (IL-2) and IL-9.
The GITR Bioassay reflects the mechanism of action (MOA) of biologics designed to activate GITR.
Control Ab, Anti-GITR, is a rabbit anti-human GITR agonist antibody. It can be used as a positive control in the GITR Bioassays (Cat.# JA2291, JA2295, J2272) in the presence of FcγRIIb CHO-K1 Cells (Cat.# J2232, JA2251, JA2255).