Promega, J2992, IL-6 Bioassay

A Simpler, More Consistent IL-6 Bioassay
Prequalified according to ICH guidelines
96- and 384-well plate formats
No primary cell culture required
Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
Specifications：
IL-6 Bioassay Cells (CPM) J299A 2 × 0.65ml
IL-6 Bioassay Cells J302A 1 × 0.65ml
Fetal Bovine Serum J121A 1 × 4ml
RPMI 1640 Medium G708A 1 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 1 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 1 × 1 vial
IL-6 Bioassay Cells J302A 5 × 0.65ml
Fetal Bovine Serum J121A 5 × 4ml
RPMI 1640 Medium G708A 5 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 5 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 5 × 1 vial
IL-6 is a member of the IL-6 cytokine family, which includes IL-11, leukemia inhibitory factor (LIF), oncostatin M, ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1) and cardiotrophin-like cytokine (CLC). All of these cytokines signal through gp130 and the STAT3 pathway. IL-6 is transiently secreted following tissue damage or stressors, including UV irradiation, reactive oxygen species, and microbial and viral agents. It is also one of the cytokines released during bacterial sepsis. Furthermore, IL-6, along with transforming growth factor β (TGFβ), promotes differentiation of CD4+ T cells into Th17 cells and inhibits differentiation of regulatory T cells, thus playing a critical role in autoimmunity.
The IL-6 Bioassay is a bioluminescent cell-based assay designed to measure IL-6 stimulation or inhibition. The IL-6 Bioassay Cells have been engineered to express luc2P in response to IL-6 signaling. When IL-6 binds to IL-6 Bioassay Cells, the receptor transduces intracellular signals, resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Reagent.