Promega, J4052, Pyruvate-Glo™ Assay

Easy, Sensitive Pyruvate Assay Kit
Compatible with cells, media and serum samples
Streamlined in-well sample preparation protocol
Scalable to 384-well plates for HTS applications
Specifications：
H G882A 1 × 40μl
Signal Enhancer Solution G883A 1 × 100μl
H G922A 1 × 2ml
Luciferin Detection Solution J135A 1 × 5ml
Neutralization Buffer J153A 1 × 15ml
0.6N HCl J402A 1 × 15ml
Pyruvate Oxidase (POX) J416A 1 × 130μl
Pyruvate, 10mM J417A 1 × 50μl
d V251A 1 × 100μl
H G882B 1 × 200μl
Signal Enhancer Solution G883B 1 × 500μl
H G922B 1 × 10ml
Luciferin Detection Solution J135B 1 × 50ml
Neutralization Buffer J153A 1 × 15ml
0.6N HCl J402A 1 × 15ml
Pyruvate Oxidase (POX) J416B 1 × 1300μl
Pyruvate, 10mM J417A 1 × 50μl
d V251B 1 × 500μl
The Pyruvate-Glo™ Assay is a fast and sensitive method for detecting pyruvate in various biological samples, such as cultured cells, media and serum. Based on bioluminescent technology, the Pyruvate-Glo™ Assay can detect subtle changes in glycolysis and mitochondrial metabolism in just 75 minutes. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation and spin columns. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.