Promega, J9030, Metabolite-Glo™ Detection System

Easy Metabolite Detection Customizable to Your Specific Targets
Bioluminescent NAD(P)H detection toolkit that can be coupled with a metabolite-specific dehydrogenase to build custom metabolite detection assays
For use with cultured cells, media samples, tissue homogenates and serum samples
Scalable to 384-well format for HTS applications
Specifications：
Reductase G884A 1 × 55μl
Reductase Substrate G885A 1 × 55μl
Luciferin Detection Solution J135A 1 × 5ml
NAD J136B 1 × 275μl
Neutralization Buffer J153A 1 × 15ml
NADP+ J154B 1 × 100μl
0.6N HCl J402A 1 × 15ml
Reductase G884B 1 × 275μl
Reductase Substrate G885B 1 × 275μl
Luciferin Detection Solution J135B 1 × 50ml
NAD J136C 1 × 1ml
Neutralization Buffer J153A 1 × 15ml
NADP+ J154C 1 × 500μl
0.6N HCl J402A 1 × 15ml
The Metabolite-Glo™ Detection System is a rapid and sensitive approach for detecting NAD(P)H in a variety of biological samples, including cultured cells, media samples, tissue homogenates and serum. This system can be optimized to detect specific metabolites by coupling NAD(P)H detection with a suitable dehydrogenase enzyme (which must be obtained separately).  We provide straightforward instructions for metabolite assay development and optimization in the technical manual.
The Metabolite-Glo™ System provides reagents for a streamlined sample preparation with acid treatment and neutralization steps performed directly in the assay wells. This eliminates the need for traditional methods such as cell collection, centrifugation and spin columns. With its sensitive detection capabilities and liquid formulation, the assay is easily adapted to a high-throughput 384-well format, enabling the efficient analysis of numerous samples simultaneously.