Promega, JA1011, RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay

Simple, Plate-Based Assay for Real-Time Apoptosis Detection
No-wash, one-step Annexin V assay
Non-lytic; allows continual monitoring of cell state to determine apoptotic onset
Scalable for high-throughput screening applications
Specifications：
Annexin V-SmBiT Reagent (1,000X) J700A 1 × 25μl
CaCl J750A 1 × 250μl
Annexin V-LgBiT Reagent (1,000X) J800A 1 × 25μl
Annexin V NanoBiT® Substrate, 1,000X JA100A 1 × 25μl
Necrosis Detection Reagent, 1,000X JA101A 1 × 25μl
Annexin V-SmBiT Reagent (1,000X) J700B 1 × 250μl
CaCl J750A 1 × 250μl
Annexin V-LgBiT Reagent (1,000X) J800B 1 × 250μl
Annexin V NanoBiT® Substrate, 1,000X JA100B 1 × 250μl
Necrosis Detection Reagent, 1,000X JA101B 1 × 250μl
Annexin V-SmBiT Reagent (1,000X) J700A 1 × 25μl
CaCl J750A 1 × 250μl
Annexin V-LgBiT Reagent (1,000X) J800A 1 × 25μl
Annexin V NanoBiT® Substrate, 1,000X JA100A 1 × 25μl
Annexin V-SmBiT Reagent (1,000X) J700B 1 × 250μl
CaCl J750A 1 × 250μl
Annexin V-LgBiT Reagent (1,000X) J800B 1 × 250μl
Annexin V NanoBiT® Substrate, 1,000X JA100B 1 × 250μl
RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay
(Cat.# JA1011 and JA1012)
Includes Necrosis Detection Reagent, a cell-impermeant profluorescent DNA dye. Upon loss of membrane integrity, the dye enters the cell and binds to DNA, generating a fluorescent signal.
RealTime-Glo™ Annexin V Apoptosis Assay
(Cat.# JA1000 and JA1001)
Does not include Necrosis Detection Reagent. Although the assay can be conducted without a necrosis detection probe, the mechanism of action of PS exposure will not be revealed. Therefore, when using Cat.# JA1000 or JA1001, we recommend incorporating an asymmetric cyanine dye such as CellTox™ Green (Cat.# G8742) or structurally related molecules, for full assay functionality and data clarity.