Promega, JA2191, OX40 Bioassay

Measure Potency and Stability of Biologics Designed to Activate OX40
Prequalified according to ICH guidelines
96- and 384-well plate formats
Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
Specifications：
OX40 Effector Cells J219A 1 × 0.5ml
Fetal Bovine Serum J121A 1 × 4ml
RPMI 1640 Medium G708A 1 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 1 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 1 × 1 vial
OX40 Effector Cells J219A 5 × 0.5ml
Fetal Bovine Serum J121A 5 × 4ml
RPMI 1640 Medium G708A 5 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 5 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 5 × 1 vial
OX40 Effector Cells (CPM) J217A 2 × 1ml
Control Ab, Anti-OX40 K119A 1 × 50μg
The OX40 Bioassay is a bioluminescent cell-based assay that measures the potency and stability of ligands or agonist antibodies that can bind and activate OX40. OX40 (CD134/TNFRSF4), a member of the tumor necrosis factor (TNF) receptor superfamily, is a costimulatory receptor expressed primarily on activated T cells, and on neutrophils and natural killer (NK) cells to a lesser extent. When present on the cell surface, OX40 interacts with OX40 ligand (OX40L) and induces subsequent cell proliferation, survival and production of cytokines, particularly in T cells.
The OX40 Bioassay reflects the mechanism of action (MOA) of biologics designed to activate OX40 following the addition of OX40 ligand or OX40 agonist antibodies.
Control Ab, Anti-OX40, is a mouse anti-human OX40 agonist antibody. It can be used as a positive control in the OX40 Bioassays in the presence of FcγRIIb CHO-K1 Cells (Cat.# J2232, JA2251, JA2255).