Promega, JA2201, IL-2 Bioassay

A Simpler, More Consistent IL-2 Bioassay
Prequalified according to ICH guidelines
Mechanism-of-action-based assay
No primary cell culture required
Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
Specifications：
IL-2 Bioassay Cells J297A 1 × 0.80ml
Fetal Bovine Serum J121A 1 × 4ml
RPMI 1640 Medium G708A 1 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 1 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 1 × 1 vial
IL-2 Bioassay Cells J297A 5 × 0.80ml
Fetal Bovine Serum J121A 5 × 4ml
RPMI 1640 Medium G708A 5 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 5 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 5 × 1 vial
IL-2 Bioassay Cells (CPM) J295A 2 × 0.75ml
Interleukin-2 (IL-2), originally described as “T-cell growth factor” in 1976, is a small 15.5kDa monomer secreted by a variety of cell types including CD4+ and CD8+ T cells, natural killer (NK) cells and activated dendritic cells. IL-2 has pleiotropic effects on the immune system. It plays a critical role in the generation, maintenance and expansion of CD4+ regulatory T cells, promotes the cytotoxic activity of NK and CD8+ cells and governs homeostasis through the elimination of harmful autoreactive T cells via activation-induced cell death.
The IL-2 Bioassay is a bioluminescent cell-based assay designed to measure IL-2 stimulation or inhibition. The assay overcomes many of the limitations of the current endothelial cell proliferation assays and can be used for the discovery and development of novel biologic therapies aimed at either inducing or inhibiting the IL-2 response. The IL-2 Bioassay Cells have been engineered to express
luc2
in response to IL-2 signaling. When IL-2 binds to IL-2 Bioassay Cells, the receptor transduces intracellular signals that result in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System (Cat.# G7940) and a standard luminometer such as the GloMax® Discover System (Cat.# GM3000).