Promega, JA2215, TIM-3 Bioassay

Measure Potency and Stability of Biologics Designed to Block TIM-3 Interactions
Uses Bio-Glo-NL™ Luciferase Assay System
96- and 384-well plate formats
No primary cell culture required
Supplied in convenient thaw-and-use format and cell propagation model (CPM) format
Specifications：
TIM-3 Effector Cells J313A 1 × 0.5ml
TIM-3 Target Cells J314A 1 × 0.9ml
Fetal Bovine Serum J121A 1 × 4ml
RPMI 1640 Medium G708A 1 × 36ml
Bio-Glo-NL™ Luciferase Assay Buffer J308A 1 × 10ml
Bio-Glo-NL™ Luciferase Assay Substrate J309A 1 × 200μl
TIM-3 Effector Cells J313A 5 × 0.5ml
TIM-3 Target Cells J314A 5 × 0.9ml
Fetal Bovine Serum J121A 5 × 4ml
RPMI 1640 Medium G708A 5 × 36ml
Bio-Glo-NL™ Luciferase Assay Buffer J308A 5 × 10ml
Bio-Glo-NL™ Luciferase Assay Substrate J309A 5 × 200μl
TIM-3 Effector Cells (CPM) J306A 2 × 1ml
TIM-3 Target Cells (CPM) J307A 2 × 1ml
Control Ab, Anti-TIM-3 K121A 1 × 100μg
The TIM-3 Bioassay is a bioluminescent reporter cell-based assay that overcomes the limitations of existing assays and can be used to measure the potency and stability of antibodies and other biologics targeting TIM-3. TIM-3 (CD366, HAVCR2) is an immune checkpoint receptor expressed on a subset of activated T cells, regulatory T cells (Tregs), macrophages and dendritic cells. TIM-3 has the capacity to inhibit or costimulate T-cell receptor (TCR) signaling in different in vitro systems.
The TIM-3 Bioassay consists of two cell lines:
When the two cell types are co-cultured, TIM-3 Target Cells provide low-level stimulation to the TIM-3 Effector Cells through the TCR. The combined stimulation of the TCR and TIM-3 induces promoter-mediated luciferase activation and luminescence. Adding a TIM-3 blocking antibody prevents TIM-3 signaling and reduces promoter-mediated luminescence.
The Control Ab, Anti-TIM-3, is a mouse anti-human TIM-3 antibody that shows blocking activity for TIM-3 with its ligands. It is intended for use as a control with the TIM-3 Bioassay, which can be used to measure the potency and stability of antibodies and other biologics designed to block the TIM-3 signaling.