Promega, JA2601, IL-12 Bioassay

A Simpler, More Consistent IL-12 Bioassay
Prequalified according to ICH guidelines
96- and 384-well plate formats
No primary cell culture required
Supplied in convenient thaw-and-use format; also available in cell propagation model (CPM) format
Specifications：
IL-12 Bioassay Cells (CPM) J304A 2 × 0.65ml
IL-12 Bioassay Cells J305A 1 × 0.65ml
Fetal Bovine Serum J121A 1 × 4ml
RPMI 1640 Medium G708A 1 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 1 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 1 × 1 vial
IL-12 Bioassay Cells J305A 5 × 0.65ml
Fetal Bovine Serum J121A 5 × 4ml
RPMI 1640 Medium G708A 5 × 36ml
Bio-Glo™ Luciferase Assay Buffer G719A 5 × 10ml
Bio-Glo™ Luciferase Assay Substrate G720A 5 × 1 vial
Interleukin-12 (IL-12) is a member of the IL-12 cytokine family, which includes IL-12, IL-23, IL-27, IL-35 and IL-39. Both IL-12 and IL-23 are composite cytokines, sharing a common IL-12 p40 subunit and IL-12Rβ1 receptor. Cytokine specificity is derived from the unique IL-12 p35 subunit binding to IL-12Rβ2 and the IL-23p19 subunit binding to IL-23R. Both p35 and p40 genes need to be expressed within the same cell to produce the active heterodimer and subsequent IL-12 signaling. IL-12 is secreted following stimulation of phagocytes and dendritic cells by bacteria and other microorganisms.
The IL-12 Bioassay is a bioluminescent cell-based assay designed to measure IL-12 stimulation or inhibition. The IL-12 Bioassay Cells have been engineered to express luc2P in response to IL-12 signaling. When IL-12 binds to IL-12 Bioassay Cells, the receptor transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Reagent.