Promega, JE9500, BHB-Glo™ (Ketone Body) Assay

Easy, Sensitive Beta-Hydroxybutyrate Assay Kit
Compatible with cells, media, tissue and serum samples
In-well sample preparation protocol streamlines workflow
Scalable to 384-well plates for HTS applications
Specifications：
β-Hydroxybutyrate Dehydrogenase J414A 1 × 100μl
β-Hydroxybutyrate, 10mM J415A 1 × 50μl
Reductase G884A 1 × 55μl
Reductase Substrate G885A 1 × 55μl
Luciferin Detection Solution J135A 1 × 5ml
NAD J136B 1 × 275μl
Neutralization Buffer J153A 1 × 15ml
NADP+ J154B 1 × 100μl
0.6N HCl J402A 1 × 15ml
β-Hydroxybutyrate Dehydrogenase J414B 1 × 1ml
β-Hydroxybutyrate, 10mM J415A 1 × 50μl
Reductase G884B 1 × 275μl
Reductase Substrate G885B 1 × 275μl
Luciferin Detection Solution J135B 1 × 50ml
NAD J136C 1 × 1ml
Neutralization Buffer J153A 1 × 15ml
NADP+ J154C 1 × 500μl
0.6N HCl J402A 1 × 15ml
The BHB-Glo™ (Ketone Body) Assay is a fast and sensitive method for detecting β-hydroxybutyrate (BHB) from various biological samples, such as cultured cells, media, tissue homogenates and serum. Based on bioluminescent technology, the BHB-Glo™ Assay can detect subtle changes in ketosis as well as ketone body consumption and secretion. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation, spin columns and deproteinization. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.