Promega, N1541, Nano-Glo® Dual-Luciferase® Reporter Assay System

Ultra-Sensitive Detection of Firefly and NanoLuc® Luciferase Activities in a Single Sample
Greater sensitivity at low expression levels
Choose either firefly or NanoLuc® luciferase as primary reporters
Improved reagent stability brings more flexibility to assay design
Specifications：
ONE-Glo™ EX Luciferase Assay Buffer E632A 1 × 10ml
ONE-Glo™ EX Luciferase Assay Substrate E633A 1 × 1 vial
NanoDLR™ Stop & Glo® Buffer N142A 1 × 10ml
NanoDLR™ Stop & Glo® Substrate N143A 1 × 100μl
ONE-Glo™ EX Luciferase Assay Buffer E632B 1 × 100ml
ONE-Glo™ EX Luciferase Assay Substrate E633B 1 × 1 vial
NanoDLR™ Stop & Glo® Buffer N142B 1 × 100ml
NanoDLR™ Stop & Glo® Substrate N143B 1 × 1ml
ONE-Glo™ EX Luciferase Assay Buffer E632A 10 × 10ml
ONE-Glo™ EX Luciferase Assay Substrate E633A 10 × 1 vial
NanoDLR™ Stop & Glo® Buffer N142A 10 × 10ml
NanoDLR™ Stop & Glo® Substrate N143A 10 × 100μl
ONE-Glo™ EX Luciferase Assay Buffer E632B 10 × 100ml
ONE-Glo™ EX Luciferase Assay Substrate E633B 10 × 1 vial
NanoDLR™ Stop & Glo® Buffer N142B 10 × 100ml
NanoDLR™ Stop & Glo® Substrate N143B 10 × 1ml
Nano-Glo® Dual-Luciferase® Reporter Assay System N1610 1 × 10ml
pNL1.1.TK[ N1501 1 × 20μg
Nano-Glo® Dual-Luciferase® Reporter Assay System N1610 1 × 10ml
pNL1.1.PGK[ N1441 1 × 20μg
Nano-Glo® Dual-Luciferase® Reporter Assay System N1610 1 × 10ml
pGL4.54[ E5061 1 × 20μg
Nano-Glo® Dual-Luciferase® Reporter Assay System N1610 1 × 10ml
pGL4.53[ E5011 1 × 20μg
The Nano-Glo® Dual-Luciferase® Reporter (NanoDLR™) Assay detects the activities of dual luciferases, firefly and NanoLuc® reporter enzymes, in a single sample. Improved firefly chemistry and the bright, sensitive NanoLuc® luciferase make this a highly sensitive dual-luciferase assay that is simple to use, provides superior data quality, and allows greater flexibility in assay design.
In the NanoDLR™ Assay protocol, firefly luciferase activity is measured first using the ONE-Glo™ EX Luciferase Assay Reagent. NanoDLR™ Stop & Glo® Reagent is then added to quench the firefly signal and provide the substrate needed to measure NanoLuc® luciferase activity.
Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.