Promega, N1662, NanoBRET® Nano-Glo® Detection Systems

Dedicated Detection Solutions for NanoBRET® Protein Interaction Assays
Measure protein interactions in live cells with full-length proteins
Use the standard reagent for single-time-point or <2-hour analysis; use the kinetic reagent for extended live-cell measurements
Increased signal and lower background compared to conventional BRET assays
Specifications：
HaloTag® NanoBRET® 618 Ligand G980A 1 × 20μl
NanoBRET® Nano-Glo® Substrate N157A 1 × 50μl
HaloTag® NanoBRET® 618 Ligand G980A 5 × 20μl
NanoBRET® Nano-Glo® Substrate N157A 5 × 50μl
HaloTag® NanoBRET® 618 Ligand G980B 1 × 1ml
NanoBRET® Nano-Glo® Substrate N157B 2 × 1.25ml
HaloTag® NanoBRET® 618 Ligand G980A 1 × 20μl
Nano-Glo® Vivazine® Substrate, 100X N258A 2 × 0.1ml
HaloTag® NanoBRET® 618 Ligand G980A 5 × 20μl
Nano-Glo® Vivazine® Substrate, 100X N258B 1 × 1ml
HaloTag® NanoBRET® 618 Ligand G980B 1 × 1ml
Nano-Glo® Vivazine® Substrate, 100X N258B 10 × 1ml
The NanoBRET® assay is a bioluminescence resonance energy transfer (BRET)-based assay that uses NanoLuc® Luciferase as the BRET energy donor and HaloTag® protein labeled with the HaloTag® NanoBRET® 618 fluorescent Ligand as the energy acceptor to measure the interaction of two binding partners in live cells.
The NanoBRET® Nano-Glo® Detection System provides the NanoBRET® Nano-Glo® Substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag® NanoBRET® 618 Ligand for the fluorescent energy acceptor. The HaloTag® NanoBRET® 618 Ligand is added directly to the cells during plating, and the NanoBRET® Nano-Glo® Substrate is added to the sample just prior to measuring donor and acceptor emission. The NanoBRET® Nano-Glo® Kinetic Detection System has the Vivazine® substrate for use by the NanoLuc® luciferase donor, giving an extended signal for live-cell kinetic assays.
For more details on using the NanoBRET® assay for protein:protein interaction studies visit:
NanoBRET® Technology for Protein Interactions
. The NanoBRET® Nano-Glo® Detection Systems are compatible with a diverse set of prebuilt or custom NanoBRET® PPI Assays.
Note:
The total number of assays will vary based on final well volume. For standard well volumes:
Cat.# N1661 and N2583 are sufficient for 200 assays in 96-well plates or 500 assays in 384-well plates.
Cat.# N1662 and N2584 are sufficient for 1,000 assays in 96-well plates or 2,500 assays in 384-well plates.
Cat.# N1663 and N2585 are sufficient for 10,000 assays in 96-well plates or 25,000 assays in 384-well plates.