Promega, N1811, NanoBRET® PPI Starter Systems

BRET-Based Assays to Detect Protein:Protein Interactions in Live Cells
Study induction and inhibition of protein interactions using full-length proteins expressed at physiologically relevant levels
Starter Systems provide fusion vectors, positive controls and detection reagents for building PPI assays
Live-cell detection options for endpoint and kinetic analysis
Specifications：
pNLF1-N [CMV/Hygro] Vector N1351 1 × 20μg
pNLF1-C [CMV/Hygro] Vector N1361 1 × 20μg
NanoBRET® PPI Control Pair (p53, MDM2) N1641 1 × 2 × 20μg
NanoBRET® Nano-Glo® Detection System N1661 1 × 200 assays
pFN31K N1321 1 × 20μg
pFC32K N1341 1 × 20μg
NanoBRET® PPI Control Pair (p53, MDM2) N1641 1 × 2 × 20μg
NanoBRET® Nano-Glo® Detection System N1661 1 × 200 assays
NanoBRET®-based protein:protein interaction (PPI) assays use NanoLuc® Luciferase as a BRET energy donor and HaloTag® protein, labeled with the NanoBRET® 618 fluorophore, as the energy acceptor to measure the interaction of specific protein pairs.
The NanoBRET® PPI Starter Systems provide the vectors required to create NanoLuc® Luciferase and HaloTag® protein fusions to target proteins of interest, a Positive Control Pair (p53, MDM2) and the NanoBRET® Nano-Glo® Detection System, which contains the substrate used by NanoLuc® Luciferase to generate the donor signal and the HaloTag® NanoBRET® 618 Ligand for the fluorescent energy acceptor.