Promega, N1830, NanoBRET® Bromodomain/Histone Interaction Assays

Protein Interaction Assays to Study BRD/Histone Interactions in Live Cells
Study full-length bromodomain/histone-containing proteins or fragments
Detect protein interactions in live cells, even at low expression levels
Choose from prebuilt or custom assay options
All but NanoBRET® BRD4/Histone H3.3 Interaction Assay are discontinued
Specifications：
HaloTag® NanoBRET® 618 Ligand G980A 2 × 20μl
NanoBRET® Nano-Glo® Substrate N157A 2 × 50μl
p53-HaloTag® Fusion Vector N159A 1 × 20μg
NanoLuc®-MDM2 Fusion Vector N160A 1 × 20μg
Histone H3.3-HaloTag® Fusion Vector N167A 1 × 20μg
NanoLuc®-BRD4 FL Fusion Vector N169A 1 × 20μg
NanoLuc®-BRD4 BD1 Fusion Vector N170A 1 × 20μg
Bromodomain (BRD)-containing proteins are critical components of nuclear protein complexes involved in the recruitment of chromatin-modifying enzymes and transcriptional regulation of acetylated chromatin. The protein:protein interaction (PPI) of the BRD-containing proteins with acetylated histones is an important method of epigenetic regulation critical for cell health and development and is of great interest for drug targeting because dysfunction in BRD modulation has been implicated as a critical event in disease formation.
The NanoBRET® Bromodomain/Histone Interaction Assays enable interaction studies of bromodomain-containing proteins with full-length histones in the context of natural chromatin. In addition to the full-length bromodomain proteins, BRD fragments alone (BRD4, BRD9 and BRPF1) are also included for users that may want to understand the interaction of this isolated domain.