Promega, N2014, NanoBiT® PPI Starter Systems

Sensitive Detection of Protein Interactions in Live Cells
Detects interactions at low expression levels and allows real-time kinetic analysis in live cells
Reversible protein complementation reporter to analyze protein association and disassociation
Improved sensitivity and accuracy over split firefly luciferase
Specifications：
pBiT1.1-C [TK/LgBiT] Vector N196A 1 × 20μg
pBiT2.1-C [TK/SmBiT] Vector N197A 1 × 20μg
pBiT1.1-N [TK/LgBiT] Vector N198A 1 × 20μg
pBiT2.1-N [TK/SmBiT] Vector N199A 1 × 20μg
NanoBiT® Negative Control Vector N202A 1 × 20μg
LgBiT-PRKAR2A Control Vector N203A 1 × 20μg
SmBiT-PRKACA Control Vector N204A 1 × 20μg
Nano-Glo® Live Cell Substrate N205A 2 × 125μl
Nano-Glo® LCS Dilution Buffer N206A 2 × 2.5ml
pFC34K LgBiT TK-neo Flexi® Vector N192A 1 × 20μg
pFC36K SmBiT TK-neo Flexi® Vector N193A 1 × 20μg
pFN33K LgBiT TK-neo Flexi® Vector N194A 1 × 20μg
pFN35K SmBiT TK-neo Flexi® Vector N195A 1 × 20μg
NanoBiT® Negative Control Vector N202A 1 × 20μg
LgBiT-PRKAR2A Control Vector N203A 1 × 20μg
SmBiT-PRKACA Control Vector N204A 1 × 20μg
Nano-Glo® Live Cell Substrate N205A 2 × 125μl
Nano-Glo® LCS Dilution Buffer N206A 2 × 2.5ml
FRB-LgBiT Control Vector N200A 1 × 20μg
FKBP-SmBiT Control Vector N201A 1 × 20μg
In contrast to many split systems, the LgBiT:SmBiT interaction is reversible, allowing the detection of rapidly dissociating proteins. PPI dynamics can be followed in real time in living cells using the Nano-Glo® Live Cell Reagent, a non-lytic detection reagent containing the cell-permeable furimazine substrate. Advantages over split systems include better sensitivity, reversibility, the ability to create fusions to peptides or small, structurally stable protein domains, and the capability for real-time measurement of protein interactions.
The poster
Monitoring Intracellular Protein Interactions using NanoBiT Binary Technology
provides more details, including data illustrating these capabilities.