Promega, N2090, NanoBRET® Target Engagement HDAC Assays

Directly Measure HDAC-Test Compound Affinity and Residence Time in Live Cells
Compound and tracer compete directly for the binding site
Assays rely on full-length proteins that are similar to the native forms
Express target proteins at low levels
Specifications：
Transfection Carrier DNA E488A 1 × 20μg
NanoBRET® Nano-Glo® Substrate N157A 1 × 50μl
Tracer Dilution Buffer N219A 1 × 5ml
NanoLuc®-HDAC6 FL Fusion Vector N223A 1 × 20μg
NanoBRET® Intracellular TE HDAC Tracer N233A 1 × 110μl
Extracellular NanoLuc® Inhibitor N235A 1 × 17μl
Transfection Carrier DNA E488B 1 × 100μg
NanoBRET® Nano-Glo® Substrate N157C 1 × 330μl
Tracer Dilution Buffer N219A 1 × 5ml
NanoLuc®-HDAC6 FL Fusion Vector N223A 1 × 20μg
NanoBRET® Intracellular TE HDAC Tracer N233B 1 × 1.1ml
Extracellular NanoLuc® Inhibitor N235B 1 × 110μl
NanoBRET® TE Intracellular HDAC Assay N2081 1 × 1,000 assays
NanoBRET® TE HDAC DNA Bundle N2120 1 × 1 each
NanoBRET® Nano-Glo® Substrate N157D 1 × 3.3ml
Tracer Dilution Buffer N219B 1 × 50ml
NanoBRET® Intracellular TE HDAC Tracer N233B 10 × 1.1ml
Extracellular NanoLuc® Inhibitor N235C 1 × 1.1ml