Promega, N5301, NanoBRET® 590 Dyes

Develop Novel NanoBRET® Tracers to Enable Target Engagement Assays for New Targets
Leverage the same NanoBRET®-branded energy acceptors used to create most Promega NanoBRET® tracers
Couple NanoBRET® 590 SE Dyes to base compounds containing a reactive amine group
Create three unique tracer structures from a common starting material using the three linker variants of the NanoBRET® 590 SE Dye
Synthesize tracers with an amine orthogonal coupling chemistry using Azide-Alkyne Click chemistry and the NanoBRET® 590 Azide Dye
Specifications：
NanoBRET® 590 SE N500A 1 × 5mg
NanoBRET® 590 SE N500B 1 × 25mg
NanoBRET® 590 Azide N510A 1 × 5mg
NanoBRET® 590 PEG(O4)-SE N520A 1 × 5mg
NanoBRET® 590 SE N500A 1 × 5mg
NanoBRET® 590 PEG(O4)-SE N520A 1 × 5mg
NanoBRET® 590 C4 SE N530A 1 × 5mg
NanoBRET® 590 C4 SE N530A 1 × 5mg
NanoBRET® 590 C4 SE N530B 1 × 25mg
The NanoBRET® Target Engagement (TE) Assay measures compound binding at select target proteins within intact cells. This target engagement assay is based on the NanoBRET® System, an energy transfer technique designed to measure molecular proximity in living cells. The NanoBRET® Target Engagement Assay analyzes the apparent affinity of test compounds by competitive displacement of a NanoBRET® tracer reversibly bound to a NanoLuc® fusion protein in a cell.
We have a number of TE assays already developed for a large number of protein targets from a variety of protein classes. If there is no existing NanoBRET® TE Assay for your target of interest, use the NanoBRET® 590 Dyes to develop novel tracers to enable NanoBRET® TE assays for new targets.
DNA-encoded libraries (DELs) provide unmatched chemical diversity and starting points for novel drug modalities. An article in
Cell Chemical Biology
presents a workflow that exploits the bifunctional attributes of DEL ligands as a platform to generate BRET probes for live-cell target-engagement studies. The combination of DEL and BRET technology accelerates hit-to-lead studies in a live-cell setting.
For more information, see: Teske, K.A.
et al.
(2023) DELs enable the development of BRET probes for target engagement studies in cells.
Cell Chem. Biol.
30
, 1–12.