Promega, ND2690, NanoBRET® Ubiquitination Starter Kit

Live-Cell BRET-Based Assay to Measure Protein Ubiquitination
General ubiquitination assay to measure changes in the relative level of target protein ubiquitination
Perform endpoint or live-cell kinetic analysis to determine protein ubiquitination dynamics
Assay ubiquitination on ectopic or endogenously expressed proteins
Specifications：
HaloTag® NanoBRET® 618 Ligand G980A 1 × 20μl
pNLF1-N [CMV/Hygro] Vector N135A 1 × 20μg
pNLF1-C [CMV/Hygro] Vector N136A 1 × 20μg
NanoBRET® Nano-Glo® Substrate N157A 1 × 50μl
NanoLuc®-BRD4 FL Fusion Vector N169A 1 × 20μg
HaloTag®-Ubiquitin Fusion Vector N272A 1 × 20μg
Regulating overall cellular protein homeostasis is critical for maintaining cell health and often altered by cellular treatments or disease states. Most proteins and their abundance are regulated via the ubiquitin proteasome system (UPS), which uses ubiquitin conjugation to signal proteins to be trafficked to the proteasome for degradation. Ubiquitination on any given target can vary in levels, mono- and poly-ubiquitination, and mediated through a variety of amino acid linkages.
The NanoBRET® Ubiquitination Starter Kit provides the tools to create target-specific live-cell ubiquitin assays that globally measure all types of ubiquitination on a target protein. The assays can be used to measure dynamic increases or decreases in the relative levels of target protein ubiquitination following cellular treatments, such as small molecules or pathway inducers, that would influence protein stabilization or degradation, respectively. These assays can be particularly useful for studying targeted degradation compounds because the bioluminescent resonance energy transfer ratio of the NanoBRET™®signal means you can investigate protein ubiquitination while simultaneously monitoring target protein levels to assess degradation.