Promega, NP4060, NanoBRET® Target Engagement K192 Kinase Selectivity System

Profile Selectivity Across a Diverse Kinase Panel in Live Cells
Profile compound cellular selectivity against a panel of 192 human kinases, broadly representing the kinome
Optimized, addition-only cellular protocol provides quantitative compound occupancy data for 192 full-length kinases in a single experiment
K192 System provides the transfection-ready, arrayed K192 Kinase Vector Panel and a single NanoBRET® TE Kinase Assay
Specifications：
NanoBRET® Nano-Glo® Substrate N157D 1 × 3.3ml
Tracer Dilution Buffer N219B 1 × 50ml
Extracellular NanoLuc® Inhibitor N235C 1 × 1.1ml
NanoBRET® Tracer K-10 N264B 10 × 300μl
NanoBRET® TE K192 Vector Plate A NP401A 2 × 1 each
NanoBRET® TE K192 Vector Plate B NP402A 2 × 1 each
Adhesive Foil Plate Seals NP4000 1 × 1 each
NanoBRET® Nano-Glo® Substrate N157D 1 × 3.3ml
Tracer Dilution Buffer N219B 1 × 50ml
Extracellular NanoLuc® Inhibitor N235C 1 × 1.1ml
NanoBRET® Tracer K-10 N264B 10 × 300μl
Transfection Carrier DNA E488B 1 × 100μg
pNL1.1.CMV[ N109A 1 × 20μg
NanoLuc®-HIPK2 Fusion Vector NV322A 1 × 20μg
NanoBRET® TE K192 Vector Plate A NP401A 2 × 1 each
NanoBRET® TE K192 Vector Plate B NP402A 2 × 1 each
Adhesive Foil Plate Seals NP4000 1 × 1 each
NanoBRET® TE K192 Vector Plate A NP401A 2 × 1 each
NanoBRET® TE K192 Vector Plate B NP402A 2 × 1 each
Adhesive Foil Plate Seals NP4000 1 × 1 each
NanoBRET® TE K192 Vector Plate A NP401A 1 × 1 each
NanoBRET® TE K192 Vector Plate B NP402A 1 × 1 each
Adhesive Foil Plate Seals NP4000 1 × 1 each
The NanoBRET® Target Engagement (TE) K192 Kinase Selectivity System can quantitatively measure intracellular compound occupancy against a panel of 192 kinases in a single experiment. The system relies on the
NanoBRET® TE technology
, a bioluminescence resonance energy transfer (BRET) method that uses the small, extremely bright NanoLuc® luciferase as the energy donor and a cell-permeable NanoBRET® TE Tracer as the energy acceptor. The K192 Kinase Selectivity System is a live-cell method using an addition-only, multiwell plate format with a single NanoBRET® TE Kinase Assay. The NanoBRET® TE K192 Kinase Selectivity System is optimized for each kinase to provide quantitative occupancy data, enabling cellular profiling of compound-kinase interactions.
Dr. Martin Matzuk explains the importance of NanoBRET® TE in their research.
See how the NanoBRET® TE K192 Kinase Selectivity System has been optimized for a 384-well plate format, allowing for higher throughput and more efficient compound screening per kit.
The Cell Chemical Biology publication is the original demonstration of how NanoBRET® TE could be used for broad kinase selectivity profiling in live cells. This publication illustrates the first live-cell method to quantitatively profile compound occupancy against hundreds of kinases.
The STAR Protocols publication improves upon the Cell Chemical Biology work and is the basis for the NanoBRET® TE K192 Kinase Selectivity System. The authors show how a single NanoBRET® TE Kinase Assay can be used to profile compound engagement across 192 full-length kinases in a single live-cell experiment using a simple workflow.