Promega, V7601, MTase-Glo™ Methyltransferase Assay

Measure Methyltransferase Activity Using a Universal Bioluminescent Assay
Simple add-and-read format
Use with any methyltransferase
No need to modify substrates
Specifications：
SAM, 1mM A120B 1 × 200μl
SAH, 15µM A233B 1 × 300μl
MTase-Glo™ Reagent, 10X V760A 1 × 1ml
MTase-Glo™ Detection Solution V761A 1 × 10ml
SAM, 1mM A120C 1 × 1ml
SAH, 15µM A233B 1 × 300μl
MTase-Glo™ Reagent, 10X V760B 1 × 5ml
MTase-Glo™ Detection Solution V761B 1 × 50ml
The MTase-Glo™ Methyltransferase Assay is a novel, bioluminescence-based assay that can be used to monitor the activities of methyltransferases (MTases) and their modulation by small molecules in many applications, including high-throughput screening.
Monitors formation of the reaction product S-adenosyl homocysteine (SAH) and can detect changes in activity of a broad range of methyltransferases, including DNA, protein, RNA and small molecule methyltransferases.
Used successfully for all classes of protein methyltransferases (lysine and arginine) and with different types of substrates (peptides, large proteins and even nucleosomes) to determine the specificity of these enzymes and their substrate requirements.