Promega, V7991, Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay

Sensitive, Bioluminescent Detection of Demethylase Activity
Easy to use add-and-read assay format
Universal assay can be used with any succinate-producing enzyme
Optimized for screening applications; low false hits
Specifications：
Succinate, 10mM V897A 1 × 50μl
Succinate-Glo™ Buffer V847A 1 × 5ml
Succinate-Glo™ Solution V894A 1 × 50μl
ATP Detection Buffer V895A 1 × 10ml
Acetoacetyl-CoA, 100X V896A 1 × 100μl
ATP Detection Substrate V898A 1 × 1 vial
Succinate, 10mM V897A 1 × 50μl
Succinate-Glo™ Buffer V847B 1 × 50ml
Succinate-Glo™ Solution V894B 1 × 500μl
ATP Detection Buffer V895B 1 × 100ml
Acetoacetyl-CoA, 100X V896B 1 × 1ml
ATP Detection Substrate V898B 1 × 1 vial
JumonjiC domain-containing histone lysine demethylases (JMJCs) play a pivotal role in determining the epigenetic status of the genome by counteracting the activities of histone lysine methyltransferases. These enzymes act as erasers by catalyzing the removal of methyl marks from specific lysine sites in histones, leading to either transcriptional repression or activation of target genes.
The Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay rapidly detects succinate formation in JumonjiC histone demethylase and Fe(II)/α-ketoglutarate-dependent dioxygenase reactions. The assay uses the reaction product, succinate, to form ATP, which drives a bioluminescent reaction to produce a signal proportional to the original demethylase/hydroxylase activity.