Promega, W1331, Lumit® Immunoassay Cellular Systems

No-Wash, One-Plate Immunoassay for Cellular Analytes
New Lumit® Lysis Buffer II included
! Offers enhanced performance across a wide range of cellular targets
New Application Notes
available for
more validated targets
Set 1: Lumit® Anti-Mouse Ab-LgBiT/Lumit® Anti-Rabbit Ab-SmBiT
Set 2: Lumit® Anti-Mouse Ab-SmBiT/Lumit® Anti-Rabbit Ab-LgBiT
Starter Kit: Includes all four Set 1 and Set 2 Lumit® Secondary Antibodies; primary antibodies supplied by the user
Specifications：
Phospho-4E-BP1 (Ser 65) mTor Set 2 (W1331)
AKT PI3K/mTOR/AKT Set 1 (W1201)
Phospho-AKT (Ser 473) PI3K/mTOR/AKT Set 1 (W1201)
BCL6 B-cell signaling Set 1 (W1201)
β-catenin (Human) WNT Set 2 (W1331)
Phospho-β-catenin (Thr 41/Ser 45) WNT Set 2 (W1331)
Burton's Tyrosine Kinase (BTK) B-cell Receptor (BCR) Set 1 (W1201)
Phospho-BTK (Tyr 223) B-cell Receptor (BCR) Set 1 (W1201)
Phospho-CHK1 (Ser 317) DDR Pathway Set 1 (W1201)
Phospho-c-Jun (Ser 63) JNK Set 1 (W1201)
Phospho-c-MET (Tyr 1234/1235) c-MET Set 1 (W1201)
Phospho-c-MET (Tyr 1349) c-MET Set 1 (W1201)
CREB PKA/CREB Set 1 (W1201)
Phospho-CREB (Ser 133) PKA/CREB Set 1 (W1201)
EGFR EGFR Set 1 (W1201)
Phospho-EGFR (Tyr 1068) EGFR Set 1 (W1201)
Phospho-EGFR (Tyr 1173) EGFR Set 1 (W1201)
ER (Estrogen Receptor) Estrogen Set 1 (W1201)
Phospho-ERK1 (Thr 202) ERK Set 1 (W1201)
Phospho-GSK1-3β (Ser 9) GSK-3β Set 1 (W1201)
Phospho-H2AX DDR Pathway Set 1 (W1201)
Phospho-HER2 (Tyr 1196) HER2 Set 1 (W1201)
Phospho-HER2 (Tyr 1221/1222) HER2 Set 1 (W1201)
IκBα NF-κB Set 1 (W1201)
Phospho-IκBα (Ser 32) NF-κB Set 1 (W1201)
Phospho-JNK (Thr 183/Tyr 185) JNK Set 1 (W1201)
p65 NF-κB Set 2 (W1331)
Phospho-p65 (Ser 536) NF-κB Set 2 (W1331)
Rb (Retinoblastoma Tumor Suppressor) Cell Cycle Set 2 (W1331)
Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 780) Cell Cycle Set 2 (W1331)
Phospho-Rb (Retinoblastoma Tumor Suppressor) (Ser 807/811) Cell Cycle Set 2 (W1331)
Ribosomal Protein S6 PI3K/mTOR/AKT Set 1 (W1201)
Phospho-Ribosomal Protein S6 (Ser 240/244) PI3K/mTOR/AKT Set 1 (W1201)
Smad1 BMP Set 1 (W1201)
Phospho-Smad1 (Ser 463/465) BMP Set 1 (W1201)
Smad2 TGF-β Set 2 (W1331)
Phospho-Smad2 (Ser 465/467) TGF-β Set 2 (W1331)
STAT1 JAK/STAT Set 1 (W1201)
Phospho-STAT1 (Tyr 701) JAK/STAT Set 1 (W1201)
Phospho-STAT1 (Ser 727) JAK/STAT Set 1 (W1201)
Phospho-STAT2 (Tyr 690) JAK/STAT Set 1 (W1201)
STAT 3 JAK/STAT Set 1 (W1201)
Phospho-STAT 3 (Tyr 705) JAK/STAT Set 1 (W1201)
AKT PROTAC MS-21 Set 1 (W1201)
B-cell lymphoma 6 protein (BCL-6) BI-3802 Set 1 (W1201)
BRD4 PROTACs ARV-771, dBET6 Set 1 (W1201)
Burton's Tyrosine Kinase (BTK) PROTAC MT-802 Set 1 (W1201)
ER (Estrogen Receptor) Fulvestrant Set 1 (W1201)
SMARCA2 (BRM) PROTAC ACBI-1 See Application Note for details
SMARCA4 (BRG1) PROTAC ACBI-1 Set 2 (W1331)
STAT3 PROTAC SD-36 Set 1 (W1201)
Digitonin G944C 1 × 50μl
Lumit® Detection Substrate C VB103A 1 × 200μl
Lumit® Immunoassay Buffer C, 10X VB115A 1 × 1.5ml
Lumit® Anti-Mouse Ab-LgBiT W102A 1 × 30μl
Lumit® Anti-Rabbit Ab-SmBiT W103A 1 × 30μl
Antibody Dilution Buffer W108A 1 × 1ml
GF-AFC Substrate G608A-C 1 × 10μl
Lumit® Lysis Buffer II, 10X VB310A 1 × 500μl
Digitonin G944D 1 × 500μl
Lumit® Detection Substrate C VB103B 2 × 1ml
Lumit® Immunoassay Buffer C, 10X VB115B 1 × 15ml
Lumit® Anti-Mouse Ab-LgBiT W102B 1 × 300μl
Lumit® Anti-Rabbit Ab-SmBiT W103B 1 × 300μl
Antibody Dilution Buffer W108A 1 × 1ml
GF-AFC Substrate G608A-C 2 × 10μl
Lumit® Lysis Buffer II, 10X VB310B 1 × 5ml
Digitonin G944D 10 × 500μl
Lumit® Detection Substrate C VB103B 20 × 1ml
Lumit® Immunoassay Buffer C, 10X VB115B 10 × 15ml
Lumit® Anti-Mouse Ab-LgBiT W102B 10 × 300μl
Lumit® Anti-Rabbit Ab-SmBiT W103B 10 × 300μl
Antibody Dilution Buffer W108A 10 × 1ml
Lumit® Lysis Buffer II, 10X VB310B 10 × 5ml
Digitonin G944C 1 × 50μl
Lumit® Detection Substrate C VB103A 1 × 200μl
Lumit® Immunoassay Buffer C, 10X VB115A 1 × 1.5ml
Lumit® Anti-Rabbit Ab-LgBiT W104A 1 × 30μl
Lumit® Anti-Mouse Ab-SmBiT W105A 1 × 30μl
Antibody Dilution Buffer W108A 1 × 1ml
GF-AFC Substrate G608A-C 1 × 10μl
Lumit® Lysis Buffer II, 10X VB310A 1 × 500μl
Digitonin G944D 1 × 500μl
Lumit® Detection Substrate C VB103B 2 × 1ml
Lumit® Immunoassay Buffer C, 10X VB115B 1 × 15ml
Lumit® Anti-Rabbit Ab-LgBiT W104B 1 × 300μl
Lumit® Anti-Mouse Ab-SmBiT W105B 1 × 300μl
Antibody Dilution Buffer W108A 1 × 1ml
GF-AFC Substrate G608A-C 2 × 10μl
Lumit® Lysis Buffer II, 10X VB310B 1 × 5ml
Digitonin G944D 10 × 500μl
Lumit® Detection Substrate C VB103B 20 × 1ml
Lumit® Immunoassay Buffer C, 10X VB115B 10 × 15ml
Lumit® Anti-Rabbit Ab-LgBiT W104B 10 × 300μl
Lumit® Anti-Mouse Ab-SmBiT W105B 10 × 300μl
Antibody Dilution Buffer W108A 10 × 1ml
Lumit® Lysis Buffer II, 10X VB310B 10 × 5ml
Digitonin G944C 2 × 50μl
Lumit® Detection Substrate C VB103A 2 × 200μl
Lumit® Immunoassay Buffer C, 10X VB115A 2 × 1.5ml
Lumit® Anti-Mouse Ab-LgBiT W102A 1 × 30μl
Lumit® Anti-Rabbit Ab-SmBiT W103A 1 × 30μl
Lumit® Anti-Rabbit Ab-LgBiT W104A 1 × 30μl
Lumit® Anti-Mouse Ab-SmBiT W105A 1 × 30μl
Antibody Dilution Buffer W108A 1 × 1ml
GF-AFC Substrate G608A-C 1 × 10μl
Lumit® Lysis Buffer II, 10X VB310A 2 × 500μl
The Lumit® Immunoassay Cellular System is a no-wash bioluminescent immunoassay that measures target analytes directly in cell lysates. The assay can be adapted to detect a wide range of proteins, phosphoproteins or small molecules of interest, provided the appropriate primary antibodies (supplied by the user) are available. We recently developed a new lysis buffer (Lumit® Lysis Buffer II), which broadens the range of cellular targets (see table below) that can be interrogated using the Lumit® Immunoassay Cellular System.
Also available are
Complete Assays
, which include sets of antibodies for several important signaling nodes—pERK, pAKT, pSTAT3, pIκBα and pBTK as well as several others. These are available as early access materials.
To save you time, we have evaluated a number of common targets using commercially available primary antibodies. We have expanded the number of Application Notes to include targets that are now accessible due to the new lysis buffer. Our collection of application notes contains protocols, example data, optimized conditions and ordering information for each primary antibody.
Note:
The total number of assays per kit will vary based on final well volume.
For standard well volumes: