Proteintech, 10004-2-Ig, Marcks Polyclonal antibody

Size: 20ul / 150ul The Marcks (10004-2-Ig) by Proteintech is a Polyclonal antibody targeting Marcks in WB, IHC, ELISA applications with reactivity to mouse samples 10004-2-Ig targets Marcks in WB, IHC, IF, IP, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IHC detected in: mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS Specification Tested Reactivity: mouse Cited Reactivity: mouse, rat, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: myristoylated alanine rich protein kinase C substrate Calculated Molecular Weight: 39 kDa Observed Molecular Weight: 88 kDa GenBank Accession Number: BC046601 Gene Symbol: Marcks Gene ID (NCBI): 17118 RRID: AB_2140311 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P26645 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.