Proteintech, 10021-1-Ig, 5 Lipoxygenase Polyclonal antibody

Size: 20ul / 150ul The 5 Lipoxygenase (10021-1-Ig) by Proteintech is a Polyclonal antibody targeting 5 Lipoxygenase in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 10021-1-Ig targets 5 Lipoxygenase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: DU 145 cells, HL-60 cells, K-562 cells, PC-3 cells, human placenta tissue Positive IHC detected in: human lung cancer tissue, human ovary tumor tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: arachidonate 5-lipoxygenase Calculated Molecular Weight: 674 aa, 78 kDa Observed Molecular Weight: 70-78 kDa GenBank Accession Number: BC130332 Gene Symbol: 5 Lipoxygenase Gene ID (NCBI): 240 RRID: AB_513237 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P09917 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.