Proteintech, 10106-1-AP, PRPF3 Polyclonal antibody

Size: 20ul / 150ul The PRPF3 (10106-1-AP) by Proteintech is a Polyclonal antibody targeting PRPF3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 10106-1-AP targets PRPF3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Y79 cells, HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The pre-mRNA splicing occurs in spliceosomes, which consist of four ribonucleoprotein (snRNP) particles (U1, U2, U5 and U4/U6) and more than fifty proteins. This U4/U6-associated splicing factor, Hprp3p, is a 77 kDa protein, which is homologous to the Saccharomyces cerevisiae splicing factor Prp3p and may play a role in spliceosome assembly. Western blot analysis of HeLa cell nuclear extracts detected PRP3 protein at an apparent molecular mass of about 90 kD(PMID: 9328476 ). The discrepancy between the molecular mass reported here and that predicted from the Hprp3p coding sequence can be attributed, at least partially, to the highly positive charge; its value of predicted isoelectric point (IP) is ∼9.99. This antibody is a rabbit polyclonal antibody raised against an internal region of human PRPF3. A predicted molecular mass of 77 kDa and the gene is named PRPF3 because it is homologous to the yeast PRP3 gene. We noticed that although the predicted molecular mass of PRPF3 is77 kDa, the protein produced in E.coli migrated like a 90 kDa protein in SDS-PAGE. (PMID: 9328476) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0154 Product name: Recombinant human PRPF3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 229-493 aa of BC000184 Sequence: MVGLANLHAMGIAPPKVELKDQTKPTPLILDEQGRTVDATGKEIELTHRMPTLKANIRAVKREQFKQQLKEKPSEDMESNTFFDPRVSIAPSQRQRRTFKFHDKGKFEKIAQRLRTKAQLEKLQAEISQAARKTGIHTSTRLALIAPKKELKEGDIPEIEWWDSYIIPNGFDLTEENPKREDYFGITNLVEHPAQLNPPVDNDTPVTLGVYLTKKEQKKLRRQTRREAQKELQEKVRLGLMPPPEPKVRISNLMRVLGTEAVQDP Predict reactive species Full Name: PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae) Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 90 kDa, 78 kDa GenBank Accession Number: BC000184 Gene Symbol: PRPF3 Gene ID (NCBI): 9129 RRID: AB_2170994 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43395 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.