Proteintech, 10114-1-AP, SNX6 Polyclonal antibody

Size: 20ul / 150ul The SNX6 (10114-1-AP) by Proteintech is a Polyclonal antibody targeting SNX6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples 10114-1-AP targets SNX6 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, RAW 264.7 cells Positive IP detected in: HepG2 cells Positive IHC detected in: mouse brain tissue, human prostate cancer tissue, human heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Sorting nexins (SNX) have previously been shown to regulate the cell-surface expression of the human epidermal growth factor receptor. On the basis of the predicted protein sequences, several members of this family, including SNX6, have been identified. SNX6, containing coiled-coil regions within its large C-terminal domain and is found distributed in both membrane and cytosolic fractions, typical of hydrophilic peripheral membrane proteins. The functions of SNX6 are likely to be unique to endosomes, mediated in part by interactions with SNX6-specific C-terminal sequences and membrane-associated determinants Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0168 Product name: Recombinant human SNX6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 19-290 aa of BC001798 Sequence: AIFKKTVAMHEVFLCRVAAHPILRRDLNFHVFLEYNQDLSVRGKNKKEKLEDFFKNMVKSADGVIVSGVKDVDDFFEHERTFLLEYHNRVKDASAKSDRMTRSHKSAADDYNRIGSSLYALGTQDSTDICKFFLKVSELFDKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKALDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELKHAKGNLQLLQNCLAVLNGDT Predict reactive species Full Name: sorting nexin 6 Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: BC001798 Gene Symbol: SNX6 Gene ID (NCBI): 58533 RRID: AB_2192720 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UNH7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.