Proteintech, 10256-1-AP, COPS6 Polyclonal antibody

Size: 20ul / 150ul The COPS6 (10256-1-AP) by Proteintech is a Polyclonal antibody targeting COPS6 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig, monkey samples 10256-1-AP targets COPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples. Tested Applications Positive WB detected in: mouse colon tissue, 3T3-L1 cells, pig lymph node tissue Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, COLO 320 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome. Specification Tested Reactivity: human, mouse, rat, pig, monkey Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0331 Product name: Recombinant human COPS6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 40-292 aa of BC002520 Sequence: SVALHPLVILNISDHWIRMRSQEGRPVQVIGALIGKQEGRNIEVMNSFELLSHTVEEKIIIDKEYYYTKEEQFKQVFKELEFLGWYTTGGPPDPSDIHVHKQVCEIIESPLFLKLNPMTKHTDLPVSVFESVIDIINGEATMLFAELTYTLATEEAERIGVDHVARMTATGSGENSTVAEHLIAQHSAIKMLHSRVKLILEYVKASEAGEVPFNHEILREAYALCHCLPVLSTDKFKTDFYDQCNDVGLMAYL Predict reactive species Full Name: COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) Calculated Molecular Weight: 327 aa, 36 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC002520 Gene Symbol: COPS6 Gene ID (NCBI): 10980 RRID: AB_2034908 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q7L5N1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.