Proteintech, 10351-1-AP, Emerin Polyclonal antibody

Size: 20ul / 150ul The Emerin (10351-1-AP) by Proteintech is a Polyclonal antibody targeting Emerin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 10351-1-AP targets Emerin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, A431 cells, COLO 320 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0236 Product name: Recombinant human EMD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 66-252 aa of BC000738 Sequence: STRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGN Predict reactive species Full Name: emerin Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC000738 Gene Symbol: EMD Gene ID (NCBI): 2010 RRID: AB_2100056 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50402 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.