Proteintech, 10356-1-AP, GGA2 Polyclonal antibody

Size: 20ul / 150ul The GGA2 (10356-1-AP) by Proteintech is a Polyclonal antibody targeting GGA2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 10356-1-AP targets GGA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, HeLa cells, mouse adipose tissue, human kidney tissue Positive IHC detected in: human colon cancer tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GGA2, also known as VEAR, belongs to the GGA protein family and regulates the trafficking of proteins between the trans-Golgi network and the lysosome. GGA2 has an amino-terminal VHS domain in its N terminus which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0370 Product name: Recombinant human GGA2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 70-316 aa of BC000284 Sequence: KIQSPQEKEALYALTVLEMCMNHCGEKFHSEVAKFRFLNELIKVLSPKYLGSWATGKVKGRVIEILFSWTVWFPEDIKIRDAYQMLKKQGIIKQDPKLPVDKILPPPSPWPKSSIFDADEEKSKLLTRLLKSNHPEDLQAANRLIKNLVKEEQEKSEKVSKRVSAVEEVRSHVKVLQEMLSMYRRPGQAPPDQEALQVVYERCEKLRPTLFRLASDTTDDDDALAEILQANDLLTQGVLLYKQVMEG Predict reactive species Full Name: golgi associated, gamma adaptin ear containing, ARF binding protein 2 Calculated Molecular Weight: 67 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: BC000284 Gene Symbol: GGA2 Gene ID (NCBI): 23062 RRID: AB_2110735 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UJY4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.