Proteintech, 10469-1-AP, RAN Polyclonal antibody

Size: 20ul / 150ul The RAN (10469-1-AP) by Proteintech is a Polyclonal antibody targeting RAN in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 10469-1-AP targets RAN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: A375 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue Positive IP detected in: mouse kidney tissue Positive IHC detected in: human breast cancer tissue, human colon tissue, human placenta tissue, human testis tissue, mouse colon tissue, mouse testis tissue, rat colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Positive FC (Intra) detected in: HeLa cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, chicken, macrobrachium rosenbergii Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0748 Product name: Recombinant human RAN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC004272 Sequence: MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL Predict reactive species Full Name: RAN, member RAS oncogene family Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 24 kDa GenBank Accession Number: BC004272 Gene Symbol: RAN Gene ID (NCBI): 5901 RRID: AB_2176484 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P62826 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.