Proteintech, 10507-2-AP, PDZK1 Polyclonal antibody

Size: 20ul / 150ul The PDZK1 (10507-2-AP) by Proteintech is a Polyclonal antibody targeting PDZK1 in WB, IP, ELISA applications with reactivity to human, mouse samples 10507-2-AP targets PDZK1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue, HEK-293 cells Positive IP detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information PDZK1 is a PDZ domain-containing membrane scaffold protein widely expressed in a variety of epithelial cells. It plays a key role in cell signal transduction and ion channel regulation by interacting with a variety of ion channels, transporters, and receptors. PDZK1 simultaneously anchors multiple membrane proteins through its PDZ domain to regulate membrane expression and activity. PDZK1 maintains cholesterol homeostasis and participates in the regulation of the urate/anion exchanger (URAT1) in renal tubular uric acid reabsorption. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0787 Product name: Recombinant human PDZK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC006496 Sequence: MTSTFNPRECKLSKQEGQNYGFFLRIEKDTEGHLVRVVEKCSPAEKAGLQDGDRVLRINGVFVDKEEHMQVVDLVRKSGNSVTLLVLDGDSYEKAVKTRVDLKELGQSQKEQGLSDNILSPVMNGGVQTWTQPRLCYLVKEGGSYGFSLKTVQGKKGVYMTDITPQGVAMRAGVLADDHLIEVNGENVEDASHEEVVEKVKKSGSRVMFLLVDKETDKRHVEQKIQFKRETASLKLLPHQPRIVEMKKGSNGYGFYLRAGSEQKGQIIKDIDSGSPAEEAGLKNNDLVVAVNGESVETLD Predict reactive species Full Name: PDZ domain containing 1 Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 63-70 kDa GenBank Accession Number: BC006496 Gene Symbol: PDZK1 Gene ID (NCBI): 5174 RRID: AB_2299495 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q5T2W1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.