Proteintech, 10546-1-AP, Alpha SNAP Polyclonal antibody

Size: 20ul / 150ul The Alpha SNAP (10546-1-AP) by Proteintech is a Polyclonal antibody targeting Alpha SNAP in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 10546-1-AP targets Alpha SNAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Background Information SNAPs (soluble NSF-attachment proteins) may play an important role in the process of docking and fusion of vesicles to their target membranes, according to the 'SNARE hypothesis'. NAPA(Alpha- SNAP) was found in a wide range of tissues and act synergistically in intra-Golgi transport. This antibody recognizes a 33kd protein in SDS page. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0836 Product name: Recombinant human NAPA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-295 aa of BC007432 Sequence: MDNSGKEAEAMALLAEAERKVKNSQSFFSGLFGGSSKIEEACEIYARAANMFKMAKNWSAAGNAFCQAAQLHLQLQSKHDAATCFVDAGNAFKKADPQEAINCLMRAIEIYTDMGRFTIAAKHHISIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANKCLLKVAGYAALLEQYQKAIDIYEQVGTNAMDSPLLKYSAKDYFFKAALCHFCIDMLNAKLAVQKYEELFPAFSDSRECKLMKKLLEAHEEQNVDSYTESVKEYDSISRLDQWLTTMLLRIKKTIQGDEEDLR Predict reactive species Full Name: N-ethylmaleimide-sensitive factor attachment protein, alpha Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC007432 Gene Symbol: Alpha SNAP Gene ID (NCBI): 8775 RRID: AB_2150721 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P54920 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.