Proteintech, 10580-1-AP, ERCC3 Polyclonal antibody

Size: 20ul / 150ul The ERCC3 (10580-1-AP) by Proteintech is a Polyclonal antibody targeting ERCC3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 10580-1-AP targets ERCC3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, A431 cells, COLO 320 cells, HT-1080 cells, mouse colon tissue Positive IP detected in: A431 cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Excision repair cross-complementing 3/xeroderma pigmentosum B (ERCC3/XPB) DNA helicase, is an essential ATP-dependent DNA helicase that functions as an integral component of the transcription factor IIH complex. ERCC3 is involved in initiation of basal transcription and global genome repair as well as in transcription-coupled repair (TCR) [PMID:19942596]. At the cellular level, a defect in ERCC3 results in high UV sensitivity, reduced post-UV RNA synthesis recovery and decreased DNA repair due to defective NER [PMID:16947863]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag0876 Product name: Recombinant human ERCC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 483-782 aa of BC008820 Sequence: LIGPKLYEANWMELQNNGYIAKVQCAEVWCPMSPEFYREYVAIKTKKRILLYTMNPNKFRACQFLIKFHERRNDKIIVFADNVFALKEYAIRLNKPYIYGPTSQGERMQILQNFKHNPKINTIFISKVGDTSFDLPEANVLIQISSHGGSRRQEAQRLGRVLRAKKGMVAEEYNAFFYSLVSQDTQEMAYSTKRQRFLVDQGYSFKVITKLAGMEEEDLAFSTKEEQQQLLQKVLAATDLDAEEEVVAGEFGSRSSQASRRFGTMSSMSGADDTVYMEYHSSRSKAPSKHVHPLFKRFRK Predict reactive species Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 3 (xeroderma pigmentosum group B complementing) Calculated Molecular Weight: 89 kDa Observed Molecular Weight: 89 kDa GenBank Accession Number: BC008820 Gene Symbol: ERCC3 Gene ID (NCBI): 2071 RRID: AB_2262174 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19447 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.