Proteintech, 10665-1-AP, ING5 Polyclonal antibody

Size: 20ul / 150ul The ING5 (10665-1-AP) by Proteintech is a Polyclonal antibody targeting ING5 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 10665-1-AP targets ING5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, PC-3 cells, mouse kidney tissue, HEK-293 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human colon cancer tissue, human ovary tumor tissue, human thyroid cancer tissue, mouse kidney tissue, rat skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information ING5, also named as p28ING5, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 is a component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Through chromatin acetylation it may regulate DNA replication and may function as a transcriptional coactivator. (PMID:12750254) Altered ING5 expression and its shift into the cytoplasm may have an impact on the malignant transformation of colorectal epithelial cells and should be considered as a biomarker for colorectal carcinogenesis. (PMID:21193223) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag1059 Product name: Recombinant human ING5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC005370 Sequence: MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLTTKPKGK Predict reactive species Full Name: inhibitor of growth family, member 5 Calculated Molecular Weight: 28 kDa Observed Molecular Weight: 28-32 kDa GenBank Accession Number: BC005370 Gene Symbol: ING5 Gene ID (NCBI): 84289 RRID: AB_2280522 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8WYH8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.